human cell lines nih 3t3 mouse embryonic fibroblasts  (ATCC)

Journal: Gels

Article Title: Tendon dECM Composited with Chitosan with Loading Skin Precursor Stem Cell Exosome for Enhanced Diabetic Wound Healing

doi: 10.3390/gels12050361

Figure Lengend Snippet: The cytocompatibility and antibacterial activity evaluation of the dECM@QCS and exo@dECM@QCS composite hydrogels. ( A ) Live cell staining was used to investigate the fibroblast proliferation (Scale = 100 μm). ( B ) Cell scratch assay was used to investigate the fibroblast migration (Scale = 100 μm). ( C ) Quantitative analysis results of the cell scratch experiment. ( D ) The CCK-8 experiment was used to detect the activity of fibroblasts. ( E ) The antibacterial properties of different materials were detected by plate counting method. “ns” indicates no statistical difference, * p < 0.05, and ** p < 0.01.

Article Snippet: The cytocompatibility of dECM@QCS and exo@dECM@QCS composite hydrogels was investigated by culturing with mouse embryonic fibroblast cells (NIH 3T3, ATCC, CRL-1658TM, Manassas, VA, USA) using fluorescent images and Cell Counting Kit-8 (CCK-8) assay [ ].

Techniques: Activity Assay, Staining, Wound Healing Assay, Migration, CCK-8 Assay

Journal: Materials Today Bio

Article Title: Feasibility of combining JAK1 gene editing via CRISPR-CasRx with EGCG–lactoferrin nanoparticle therapy in a microneedle-based platform for atopic dermatitis

doi: 10.1016/j.mtbio.2026.102884

Figure Lengend Snippet: Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).

Article Snippet: Mouse embryonic fibroblast NIH/3T3 cells and mouse dendritic DC2.4 cells were obtained from the American Type Culture Collection (ATCC).

Techniques: Plasmid Preparation, Zeta Potential Analyzer, Agarose Gel Electrophoresis, Transmission Assay, Electron Microscopy, Fluorescence, Transfection, Expressing

Journal: Cells

Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment

doi: 10.3390/cells15050429

Figure Lengend Snippet: Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.

Article Snippet: The mouse embryonic fibroblast cell line NIH/3T3 (ATCC; LGC Standards GmbH, Wesel, Germany) was used as a negative control in this study.

Techniques: