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human cell lines nih 3t3 mouse embryonic fibroblasts  (ATCC)


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    ATCC human cell lines nih 3t3 mouse embryonic fibroblasts
    Human Cell Lines Nih 3t3 Mouse Embryonic Fibroblasts, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 13151 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+embryonic+fibroblast+nih+3t3+cells/NIH%2F3T3/pm42229414-756-2-9
    Average 99 stars, based on 13151 article reviews
    human cell lines nih 3t3 mouse embryonic fibroblasts - by Bioz Stars, 2026-09
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    Plasmid Preparation:

    Article Title: Desert Hedgehog Down-regulation Mediates Inhibition of Proliferation by γ-Glutamylcyclotransferase Knockdown in Murine Glioblastoma Stem Cells
    Article Snippet: .. Mouse embryonic fibroblast NIH-3T3 cells were purchased from the American Type Culture Collection (Rockville, MD, USA) and GGCT was overexpressed using pCX4bsr vector as described in ( , ). .. NIH-3T3 cells were maintained in Dulbecco’s modified Eagle’s medium (Wako Pure Chemical Industries, Osaka, Japan) supplemented with 10% or 2% fetal bovine serum and 25 mM HEPES.

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. The NIH/3T3-huFAP cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. The NIH/3T3-huFAP cells or NIH/3T3-wt cells were irradiated with 27 Gy using a RS 2000 irradiator (Rad Source Technologies to prevent the cells from proliferating.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: TABLE 62 EC50 values of binding to activated human CD8 T cells Clone EC50 [nM] 25G7 11.14 25G7/FAP 28H1 2 + 2 13.21 25G7/DP47 2 + 2 50.03 12B3 0.43 12B3/FAP 28H1 2 + 2 1.99 11D5 1.35 11D5/FAP 28H1 2 + 2 7.91 9.2.2.2 Binding to Human FAP-Expressing Tumor Cells For binding assays on cell-surface expressed Fibroblast Activation Protein (FAP) NIH/3T3-huFAP clone 19 cell line or human melanoma cell line WM-266-4 (ATCC CRL-1676) were used. .. NIH/3T3-huFAP clone 19 was generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. The cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).

    Cell Culture:

    Article Title: Triterpenoid derivatives inhibit Gli-mediated transcription in human glioblastoma cell line via direct interaction with Gli1.
    Article Snippet: .. Cell lines, chemicals, and antibodies Human glioblastoma U-87MG cells (HTB-14), human lung carcinoma A-549 cells (CCL-185), and mouse embryonic fibroblast NIH 3T3 cells (CRL-1658) were purchased from the American Type Culture Collection (ATCC, Manassas, USA) and cultured in DMEM medium (Sigma-Aldrich) supplemented with 10 % of FCS (SigmaAldrich) in a CO2 incubator at 37 °C. .. For the cell-based reporter assay, U-87MG cells Jo urn al Pr e-p roo f 18 were transferred to serum reduced OptiMEM medium (Invitrogen) supplemented with 0.5 % of FCS, 1 mM of sodium pyruvate (HyClone) and 1× concentrated non-essential amino acid solution (HyClone).

    Article Title: Triterpenoid derivatives inhibit Gli-mediated transcription in human glioblastoma cell line via direct interaction with Gli1
    Article Snippet: .. Human glioblastoma U-87MG cells (HTB-14), human lung carcinoma A-549 cells (CCL-185), and mouse embryonic fibroblast NIH 3T3 cells (CRL-1658) were purchased from the American Type Culture Collection (ATCC) and cultured in DMEM medium (Sigma-Aldrich) supplemented with 10% of FCS (Sigma-Aldrich) in a CO 2 incubator at 37 °C. .. For the cell-based reporter assay, U-87MG cells were transferred to serum reduced OptiMEM medium (Invitrogen) supplemented with 0.5% of FCS, 1 mM of sodium pyruvate (HyClone) and 1× concentrated non-essential amino acid solution (HyClone).

    Generated:

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. The NIH/3T3-huFAP cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. The NIH/3T3-huFAP cells or NIH/3T3-wt cells were irradiated with 27 Gy using a RS 2000 irradiator (Rad Source Technologies to prevent the cells from proliferating.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: TABLE 62 EC50 values of binding to activated human CD8 T cells Clone EC50 [nM] 25G7 11.14 25G7/FAP 28H1 2 + 2 13.21 25G7/DP47 2 + 2 50.03 12B3 0.43 12B3/FAP 28H1 2 + 2 1.99 11D5 1.35 11D5/FAP 28H1 2 + 2 7.91 9.2.2.2 Binding to Human FAP-Expressing Tumor Cells For binding assays on cell-surface expressed Fibroblast Activation Protein (FAP) NIH/3T3-huFAP clone 19 cell line or human melanoma cell line WM-266-4 (ATCC CRL-1676) were used. .. NIH/3T3-huFAP clone 19 was generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. The cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).

    Transfection:

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. The NIH/3T3-huFAP cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. The NIH/3T3-huFAP cells or NIH/3T3-wt cells were irradiated with 27 Gy using a RS 2000 irradiator (Rad Source Technologies to prevent the cells from proliferating.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: TABLE 62 EC50 values of binding to activated human CD8 T cells Clone EC50 [nM] 25G7 11.14 25G7/FAP 28H1 2 + 2 13.21 25G7/DP47 2 + 2 50.03 12B3 0.43 12B3/FAP 28H1 2 + 2 1.99 11D5 1.35 11D5/FAP 28H1 2 + 2 7.91 9.2.2.2 Binding to Human FAP-Expressing Tumor Cells For binding assays on cell-surface expressed Fibroblast Activation Protein (FAP) NIH/3T3-huFAP clone 19 cell line or human melanoma cell line WM-266-4 (ATCC CRL-1676) were used. .. NIH/3T3-huFAP clone 19 was generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. The cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).

    Expressing:

    Article Title: Bispecific antigen binding molecules capable of specific binding to CD40 and to fap
    Article Snippet: .. The NIH/3T3-huFAP cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. The NIH/3T3-huFAP cells or NIH/3T3-wt cells were irradiated with 27 Gy using a RS 2000 irradiator (Rad Source Technologies to prevent the cells from proliferating.

    Article Title: Bispecific antibodies specific for OX40
    Article Snippet: TABLE 62 EC50 values of binding to activated human CD8 T cells Clone EC50 [nM] 25G7 11.14 25G7/FAP 28H1 2 + 2 13.21 25G7/DP47 2 + 2 50.03 12B3 0.43 12B3/FAP 28H1 2 + 2 1.99 11D5 1.35 11D5/FAP 28H1 2 + 2 7.91 9.2.2.2 Binding to Human FAP-Expressing Tumor Cells For binding assays on cell-surface expressed Fibroblast Activation Protein (FAP) NIH/3T3-huFAP clone 19 cell line or human melanoma cell line WM-266-4 (ATCC CRL-1676) were used. .. NIH/3T3-huFAP clone 19 was generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).

    Article Title: Methods of treating cancer by administering tumor necrosis factor (TNF) family ligand trimer-containing antigen-binding molecules
    Article Snippet: .. The cells were generated by transfection of mouse embryonic fibroblast NIH/3T3 cells (ATCC CRL-1658) with the expression pETR4921 plasmid encoding human FAP under a CMV promoter. .. Cells were maintained in the presence of 1.5 μg/mL puromycin (InvivoGen, Cat.-No.: ant-pr-5).



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    Image Search Results


    The cytocompatibility and antibacterial activity evaluation of the dECM@QCS and exo@dECM@QCS composite hydrogels. ( A ) Live cell staining was used to investigate the fibroblast proliferation (Scale = 100 μm). ( B ) Cell scratch assay was used to investigate the fibroblast migration (Scale = 100 μm). ( C ) Quantitative analysis results of the cell scratch experiment. ( D ) The CCK-8 experiment was used to detect the activity of fibroblasts. ( E ) The antibacterial properties of different materials were detected by plate counting method. “ns” indicates no statistical difference, * p < 0.05, and ** p < 0.01.

    Journal: Gels

    Article Title: Tendon dECM Composited with Chitosan with Loading Skin Precursor Stem Cell Exosome for Enhanced Diabetic Wound Healing

    doi: 10.3390/gels12050361

    Figure Lengend Snippet: The cytocompatibility and antibacterial activity evaluation of the dECM@QCS and exo@dECM@QCS composite hydrogels. ( A ) Live cell staining was used to investigate the fibroblast proliferation (Scale = 100 μm). ( B ) Cell scratch assay was used to investigate the fibroblast migration (Scale = 100 μm). ( C ) Quantitative analysis results of the cell scratch experiment. ( D ) The CCK-8 experiment was used to detect the activity of fibroblasts. ( E ) The antibacterial properties of different materials were detected by plate counting method. “ns” indicates no statistical difference, * p < 0.05, and ** p < 0.01.

    Article Snippet: The cytocompatibility of dECM@QCS and exo@dECM@QCS composite hydrogels was investigated by culturing with mouse embryonic fibroblast cells (NIH 3T3, ATCC, CRL-1658TM, Manassas, VA, USA) using fluorescent images and Cell Counting Kit-8 (CCK-8) assay [ ].

    Techniques: Activity Assay, Staining, Wound Healing Assay, Migration, CCK-8 Assay

    Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).

    Journal: Materials Today Bio

    Article Title: Feasibility of combining JAK1 gene editing via CRISPR-CasRx with EGCG–lactoferrin nanoparticle therapy in a microneedle-based platform for atopic dermatitis

    doi: 10.1016/j.mtbio.2026.102884

    Figure Lengend Snippet: Characterization and gene editing efficiency of PBAE-Plasmid NPs. ( a ) and ( b ) RNA silencing effects of different sgRNAs targeting JAK1 in NIH-3T3 and DC 2.4 cells. ( c ) Size and zeta potential of the PBAE-plasmid complex at various mass ratios. ( d ) Agarose gel electrophoresis of the PBAE/plasmid complex at different mass ratios. ( e ) Size distribution analyzed by dynamic light scattering (DLS) and transmission electron microscopy (TEM) images of PBAE-plasmid NPs at a mass ratio of 20:1. ( f ) and ( g ) Effects of the PBAE-plasmid complex at various mass ratios on NIH-3T3 and DC 2.4 cell viability. ( h ) Green fluorescence in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( i ) JAK1 mRNA expression in NIH-3T3 cells transfected with PBAE-plasmid NPs. ( j ) JAK1 protein expression in mice transfected with PBAE-plasmid NPs. ( k ) Quantitative analysis of (j). Data are presented as mean ± SD (n = 3). Bars sharing the same letter are not significantly different, whereas those with different letters are statistically significant (p < 0.05).

    Article Snippet: Mouse embryonic fibroblast NIH/3T3 cells and mouse dendritic DC2.4 cells were obtained from the American Type Culture Collection (ATCC).

    Techniques: Plasmid Preparation, Zeta Potential Analyzer, Agarose Gel Electrophoresis, Transmission Assay, Electron Microscopy, Fluorescence, Transfection, Expressing

    Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.

    Journal: Cells

    Article Title: Preclinical Tumorigenicity Study of an Advanced Therapy Medicinal Product for Diffuse Cartilage Lesions in an Osteoarthritic Environment

    doi: 10.3390/cells15050429

    Figure Lengend Snippet: Representative image of the subcutaneous implantation site of the NIH/3T3 group. H&E shows a large subcutaneous mass and ink in the middle. The upper left corner shows a detail of the ink-bearing cells. The picture was taken at 10×; 80 µm. Close-up picture (upper left corner) was taken at 40×; 20 µm.

    Article Snippet: The mouse embryonic fibroblast cell line NIH/3T3 (ATCC; LGC Standards GmbH, Wesel, Germany) was used as a negative control in this study.

    Techniques: